Ephrin A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Ephrin A3 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12480-1-AP

Synonyme

EFL 2, EFL2, EFNA3, Ehk1 L, EHK1 ligand, ephrin A3, EPLG3, LERK 3, LERK3

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 12480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12480-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12480-1-AP

  IP Result of anti-Ephrin A3 (IP:12480-1-AP, 4ug; Detection:12480-1-AP 1:500) with A549 cells lysate 1040ug.

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  IHC staining of human skeletal muscle using 12480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 12480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 12480-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Ephrin A3 (12480-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

12480-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Ephrin A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ephrin A3 fusion protein Ag3188
• Vollständiger Name: ephrin-A3
• Berechnetes Molekulargewicht: 238 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-35 kDa
• GenBank-Zugangsnummer: BC017722
• Gene symbol: EFNA3
• Gene ID (NCBI): 1944
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Med; EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma.; Authors - Ruzetuoheti Yiminniyaze
human	WB	Biomed Res Int; OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.; Authors - Hui Wang ; KD Validated
human	WB,IF	J Exp Clin Cancer Res; The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells; Authors - Marianna Talia ; KD Validated
rat	WB	Brain Res Bull; Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats; Authors - Tao Tong