EGF Polyklonaler Antikörper

EGF Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27141-1-AP

Synonyme

Pro-epidermal growth factor

---

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

27141-1-AP bindet in WB, IHC, ELISA EGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EGF fusion protein Ag25997
• Vollständiger Name: epidermal growth factor (beta-urogastrone)
• Berechnetes Molekulargewicht: 1207 aa, 134 kDa
• GenBank-Zugangsnummer: BC093731
• Gene symbol: EGF
• Gene ID (NCBI): 1950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal Growth Factor (EGF) is a type of mitogenic factor that stimulates the proliferation of various cells, including epithelial cells and fibroblasts. It is a small peptide composed of 53 amino acids with a molecular weight of approximately 6,000 Daltons. EGF contains three disulfide bonds, which contribute to its stability under acidic and high-temperature conditions. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF plays a crucial role in regulating cell growth, proliferation, and differentiation. When EGF binds to its receptor (EGFR), it activates intracellular signaling pathways that lead to DNA synthesis and cell proliferation. EGF is naturally present in various tissues and body fluids, including saliva, urine, and milk. It is primarily synthesized in the submandibular glands and duodenum.

Publikationen

Species	Application	Title
human	WB,IHC	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
		Genes (Basel); Evolutionary and Association Analysis of Buffalo FABP Family Genes Reveal Their Potential Role in Milk Performance.; Authors - Tingzhu Ye
human	WB	J Ethnopharmacol; Proteomics analysis of tumor exosomes reveals vital pathways of Jinfukang inhibiting circulating tumor cells metastasis in lung cancer.; Authors - Zu-Jun Que
human	IHC	Int J Exp Pathol; Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma; Authors - Chao Zeng
human	WB	Evid Based Complement Alternat Med; Evodiamine Inhibits Gastric Cancer Cell Proliferation via PTEN-Mediated EGF/PI3K Signaling Pathway.; Authors - Ruichuang Yang
human	WB	ACS Appl Mater Interfaces; Paper/PMMA Hybrid 3D Cell Culture Microfluidic Platform for the Study of Cellular Crosstalk; Authors - Kin Fong Lei