EGF Rekombinanter Antikörper

EGF Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242000B1

Kat-Nr. : 84601-1-PBS

Synonyme

242000B1, Epidermal growth factor, Pro-epidermal growth factor

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  WB analysis of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84601-1-RR (EGF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84601-1-RR (EGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84601-1-RR (EGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 84601-1-RR (same clone as 84601-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using EGF antibody (84601-1-RR, Clone: 242000B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84601-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01451-1

  Cytometric bead array standard curve of MP01451-1, MOUSE EGF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84601-1-PBS. Detection antibody: 84601-2-PBS. Standard: Eg1965. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84601-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84601-1-PBS against Mouse EGF was performed. The affinity constant is 0.671 nM.

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Geprüfte Anwendungen

Produktinformation

84601-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA EGF und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EGF fusion protein Eg1965
• Vollständiger Name: epidermal growth factor
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_010113
• Gene symbol: Egf
• Gene ID (NCBI): 13645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal Growth Factor (EGF) is a type of mitogenic factor that stimulates the proliferation of various cells, including epithelial cells and fibroblasts. It is a small peptide composed of 53 amino acids with a molecular weight of approximately 6,000 Daltons. EGF contains three disulfide bonds, which contribute to its stability under acidic and high-temperature conditions. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF plays a crucial role in regulating cell growth, proliferation, and differentiation. When EGF binds to its receptor (EGFR), it activates intracellular signaling pathways that lead to DNA synthesis and cell proliferation. EGF is naturally present in various tissues and body fluids, including saliva, urine, and milk. It is primarily synthesized in the submandibular glands and duodenum.