EGFR Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EGFR Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A2H10

Kat-Nr. : 66455-1-PBS

Synonyme

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2A2H10

---

• 

  WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66455-1-Ig (same clone as 66455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)

  WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of EC109 using 66455-1-Ig (same clone as 66455-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66455-1-Ig (same clone as 66455-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66455-1-Ig (same clone as 66455-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66455-1-Ig (same clone as 66455-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 66455-1-Ig (same clone as 66455-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 66455-1-Ig (same clone as 66455-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66455-1-Ig (same clone as 66455-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66455-1-Ig (same clone as 66455-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66455-1-Ig (same clone as 66455-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66455-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66455-1-PBS bindet in WB, IHC, Indirect ELISA EGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EGFR fusion protein Ag24947
• Vollständiger Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 1210 aa, 134 kDa
• Beobachtetes Molekulargewicht: 145-165 kDa
• GenBank-Zugangsnummer: BC094761
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.