EGFR Monoklonaler Antikörper

EGFR Monoklonal Antikörper für WB, IF/ICC, FC, ELISA, Blocking

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC, ELISA, Blocking

Konjugation

Unkonjugiert

CloneNo.

2C8C12

Kat-Nr. : 68643-1-Ig

Synonyme

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R, 2C8C12

---

• 

  WB analysis of SCaBER using 68643-1-Ig

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-468 using 68643-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68643-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 68643-1-Ig

  Immunofluorescent analysis of un-fixed A431 cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of HaCaT using 68643-1-Ig

  Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of A431 using 68643-1-Ig

  1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed.

• 

  Blocking experiment of HeLa cells using 68643-1-Ig

  Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SCaBER cells, A431-Zellen, HaCaT-Zellen, MDA-MB-468-Zellen
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen
• Erfolgreiche Detektion in Blocking: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension
• BLOCKING: BLOCKING : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68643-1-Ig bindet in WB, IF/ICC, FC, ELISA, Blocking EGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EGFR fusion protein Ag24947
• Vollständiger Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 134kd
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_005228.5
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).