EGFR Monoklonaler Antikörper

EGFR Monoklonal Antikörper für WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test

Konjugation

Unkonjugiert

CloneNo.

2C8C12

Kat-Nr. : 68643-1-PBS

Synonyme

2C8C12, EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1

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  WB analysis of SCaBER using 68643-1-Ig (same clone as 68643-1-PBS)

  SCaBER cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-468 using 68643-1-Ig (same clone as 68643-1-PBS)

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  WB analysis using 68643-1-Ig (same clone as 68643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68643-1-Ig (EGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50069-1

  Sandwich ELISA standard curve of MP50069-1, EGFR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68643-1-PBS. Detection antibody: 68643-2-PBS. Standard: Eg0204. Range: 0.156-10 ng/mL.

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  Sample test of MP50069-1

  Serum of sixteen individual healthy human donors was measured. The human EGFR concentration of detected samples was determined to be 36.08 ng/mL with a range of 23.43 - 61.95 ng/mL.

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  Sample test of MP50069-1

  HT-29 and MDA-MB-231 were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the culture supernates were removed, assayed for human IDO1, and measured 0.31 ng/mL and 1.12 ng/mL.

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  Sample test of MP50069-1

  Human milk of seven individual healthy human donors was measured. The human EGFR concentration of detected samples was determined to be 1.03 ng/mL with a range of 0.16 - 1.72 ng/mL.

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  Sample test of MP50069-1

  The mean EGFR concentration was determined to be 250.71 ng/mL in HeLa cell extract based on a 4.4 mg/mL extract load and 2.64 ng/mL in HepG2 cell extract based on a 4.1 mg/mL extract load.

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  IF Staining of HaCaT using 68643-1-Ig (same clone as 68643-1-PBS)

  Immunofluorescent analysis of un-fixed HaCaT cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

  Immunofluorescent analysis of un-fixed A431 cells using EGFR antibody (68643-1-Ig, Clone: 2C8C12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 68643-1-Ig (same clone as 68643-1-PBS)

  1x10^6 A431 cells were surface stained with 0.2 μg EGFR Monoclonal Antibody (68643-1-Ig, Clone:2C8C12) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(red), or 0.2 μg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were not fixed. This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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  Blocking experiment of HeLa cells using 68643-1-Ig (same clone as 68643-1-PBS)

  Human EGF (Cat.NO. HZ-1326 ) stimulates proliferation of HeLa cells in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human EGF (Cat.NO. HZ-1326 ) is blocked by mouse anti-human EGFR monoclonal antibody 68643-1-Ig at serial dose (red curve, refer to top X-right Y axis). The EC50 is typically 0.2-0.8 μg/mL at the presence of 1ng/ml EGF (Cat.NO. HZ-1326 ). This data was developed using the same antibody clone with 68643-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68643-1-PBS bindet in WB, IF/ICC, FC, Sandwich ELISA, Indirect ELISA, Blocking, Sample test EGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EGFR fusion protein Ag24947
• Vollständiger Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 134kd
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_005228.5
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGFR, also named ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. When stained with live cells, EGFR can be transported into the cell interior, which is consistent with the literature report (PMID: 18084617).