PHD2/EGLN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PHD2/EGLN1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 19886-1-AP

Synonyme

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

---

• 

  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19886-1-AP

  IP result of anti-PHD2/EGLN1 (IP:19886-1-AP, 4ug; Detection:19886-1-AP 1:5000) with HEK-293 cells lysate 1280 ug.

• 

  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 19886-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PHD2/EGLN1 antibody (19886-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HepG2-Zellen, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

19886-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PHD2/EGLN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHD2/EGLN1 fusion protein Ag13706
• Vollständiger Name: egl nine homolog 1 (C. elegans)
• Berechnetes Molekulargewicht: 426 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC005369
• Gene symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao ; KO Validated
mouse	WB	Adv Sci (Weinh); Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway; Authors - Ling-Na Zhao
	WB,IHC	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
	WB	Cell Mol Life Sci; Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.; Authors - Chang-Chun Song
human	WB	Int J Mol Sci; Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α.; Authors - Kenta Kaihara
mouse	WB	Biomed Pharmacother; A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.; Authors - Shilei Zhang