PHD2/EGLN1 Polyklonaler Antikörper

PHD2/EGLN1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20368-1-AP

Synonyme

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

---

• 

  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 20368-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20368-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PHD2/EGLN1 antibody (20368-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

20368-1-AP bindet in WB, IHC, IF/ICC, ELISA PHD2/EGLN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: egl nine homolog 1 (C. elegans)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 36 kDa
• GenBank-Zugangsnummer: NM_022051
• Gene symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Publikationen

Species	Application	Title
human	WB	Oncogene; iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.; Authors - Dong Zhao
mouse	WB,IHC	Am J Pathol; Genetic Loss of HIF-Prolyl-Hydroxylase 1, but Not Pharmacological Inhibition, Mitigates Hepatic Fibrosis; Authors - Christopher Tuffs
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang