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EGLN3 Polyklonaler Antikörper

EGLN3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27875-1-PBS

Synonyme

EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3

Filter:
  • Western Blot (WB) analysis of various lysates using EGLN3 Polyclonal antibody (27875-1-AP)

    WB analysis using 27875-1-AP (same clone as 27875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EGLN3 Polyclonal antibody (27875-1-AP)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27875-1-PBS bindet in WB, IHC, Indirect ELISA EGLN3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EGLN3 fusion protein Ag27464
Vollständiger Name egl nine homolog 3 (C. elegans)
Berechnetes Molekulargewicht 27 kDa
Beobachtetes Molekulargewicht27 kDa
GenBank-ZugangsnummerBC010992
Gene symbol EGLN3
Gene ID (NCBI) 112399
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.