EGR1 Polyklonaler Antikörper

EGR1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31098-1-AP

Synonyme

AT225, early growth response 1, EGR 1, EGR1, G0S30, KROX 24, KROX24, NGFI A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, Zinc finger protein 225, Zinc finger protein Krox 24, ZNF225



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

31098-1-AP bindet in WB, ELISA EGR1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EGR1 fusion protein Ag33440
Vollständiger Name early growth response 1
Berechnetes Molekulargewicht 543 aa, 58 kDa
Beobachtetes Molekulargewicht 70-80 kDa
GenBank-ZugangsnummerBC073983
Gene symbol EGR1
Gene ID (NCBI) 1958
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGR1, also named as Early growth response protein 1, is a 543 amino acid protein, which belongs to the EGR C2H2-type zinc-finger protein family. EGR1 as a transcriptional regulator recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes and binds double-stranded target DNA, irrespective of the cytosine methylation status. EGR1 regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. EGR1 activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation and plays a role in the regulation of cell survival, proliferation and cell death. Egr1 is suggested to be a 55-kDa protein according to the translation of its coding sequence. Analysis of cytoplasmic and nuclear fractions of normal and irradiated native CNS tissue by Western blotting revealed a 110-kDa band for Egr1 localized in the cytoplasm and a 75-kDa version for the nuclear Egr1(PMID: 17497096)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for EGR1 antibody 31098-1-APProtokoll herunterladen
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