EHD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EHD2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (2)

Kat-Nr. : 11440-1-AP

Synonyme

EH domain containing 2, EH domain containing protein 2, EHD2, PAST homolog 2, PAST2

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Galerie der Validierungsdaten

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  WB analysis using 11440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11440-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11440-1-AP

  IP Result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11440-1-AP bindet in WB, IP, IHC, IF, ELISA EHD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EHD2 fusion protein Ag2002
• Vollständiger Name: EH-domain containing 2
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014445
• Gene symbol: EHD2
• Gene ID (NCBI): 30846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Am J Cancer Res; Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.; Authors - Junqiang Li ; KD Validated
human	IF	PLoS One; Super-resolution analysis of PACSIN2 and EHD2 at caveolae.; Authors - Tamako Nishimura
human	IHC	Cancer Invest; EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer.; Authors - Chengqi Guan
	WB	Cancer Invest; Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach.; Authors - Jingjing Lv
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Laura (Verified Customer) (01-27-2022)

It works well for western blot with different bands but one specific around 65 kDa. It also works well for immunofluorescence of cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000WB 1/100IF
• Cell Tissue Type: MOVAS (Mouse Primary vascular aortic smooth muscle cells) and RPE-1 (Human retinal pigment epithelial-1)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs sample for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs