EHD2 Polyklonaler Antikörper

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• KD/KO Validated

EHD2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11440-1-PBS

Synonyme

PAST2, PAST homolog 2, EH domain-containing protein 2, EH domain containing protein 2, EH domain containing 2

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  WB analysis using 11440-1-AP (same clone as 11440-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11440-1-AP (same clone as 11440-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 11440-1-AP (same clone as 11440-1-PBS)

  IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11440-1-AP (same clone as 11440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11440-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11440-1-PBS bindet in WB, IHC, IP, Indirect ELISA EHD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EHD2 fusion protein Ag2002
• Vollständiger Name: EH-domain containing 2
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014445
• Gene symbol: EHD2
• Gene ID (NCBI): 30846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.