EHD2 Rekombinanter Antikörper

EHD2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243038C6

Kat-Nr. : 85604-4-RR

Synonyme

EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2

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  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85604-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85604-4-RR (EHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85604-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85604-4-RR against Human EHD2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, MDA-MB-231-Zellen, Mausherzgewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85604-4-RR bindet in WB, ELISA EHD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EHD2 fusion protein Ag2002
• Vollständiger Name: EH-domain containing 2
• Berechnetes Molekulargewicht: 543 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC014445
• Gene symbol: EHD2
• Gene ID (NCBI): 30846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.