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EHMT1 Polyklonaler Antikörper

EHMT1 Polyklonal Antikörper für IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55436-1-PBS

Synonyme

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  • Immunoprecipitation (IP) experiment of K-562 cells using EHMT1 Polyclonal antibody (55436-1-AP)

    IP experiment of K-562 using 55436-1-AP (same clone as 55436-1-PBS)

    IP result of anti-EHMT1 (IP:55436-1-AP, 4ug; Detection:55436-1-AP 1:500) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 55436-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EHMT1 Polyclonal antibody (55436-1-AP)

    IF Staining of HeLa using 55436-1-AP (same clone as 55436-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EHMT1 antibody (55436-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55436-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

55436-1-PBS bindet in IF/ICC, IP, Indirect ELISA EHMT1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name euchromatic histone-lysine N-methyltransferase 1
Berechnetes Molekulargewicht 141 kDa
Beobachtetes Molekulargewicht150-170 kDa
GenBank-ZugangsnummerNM_024757
Gene symbol EHMT1
Gene ID (NCBI) 79813
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EHMT1, also known as GLP (G9a-like protein), encodes a histone lysine methyltransferase that controls brown adipose cell fate, adaptive thermogenesis, and glucose homeostasis in vivo (PMID: 24196706). EHMTs, along with LSD1 (an H3K4 demethylase), are components of the REST complex, which silences neuronal pathway genes in non-neuronal cells. EHMT1 is mainly responsible for the monomethylation and dimethylation of histone H3 lysine 9 (H3K9), forming a heteromeric complex with EHMT2 (G9a) in euchromatin (PMID: 34214254, 37663929).