EHMT2/G9a Polyklonaler Antikörper

EHMT2/G9a Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11595-1-AP

Synonyme

BAT8, C6orf30, EHMT2, EHMT2/G9a, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

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  WB analysis of Jurkat using 11595-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11595-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11595-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11595-1-AP (EHMT2/G9a antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

11595-1-AP bindet in WB, ELISA EHMT2/G9a und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EHMT2/G9a fusion protein Ag2171
• Vollständiger Name: euchromatic histone-lysine N-methyltransferase 2
• Berechnetes Molekulargewicht: 1210 aa, 132 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC018718
• Gene symbol: EHMT2
• Gene ID (NCBI): 10919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Exp Gerontol; Age-related changes in histone modification in rat gastrocnemius muscle.; Authors - Toshinori Yoshihara
human	WB	Mol Cell Biochem; Histone methyltransferase G9a and H3K9 dimethylation inhibit the self-renewal of glioma cancer stem cells.; Authors - Hong Tao
human	WB	Cell Rep; Branched-Chain Amino Acid Metabolic Reprogramming Orchestrates Drug Resistance to EGFR Tyrosine Kinase Inhibitors.; Authors - Yuetong Wang ; KD Validated