EI24 Polyklonaler Antikörper

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EI24 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20456-1-PBS

Synonyme

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  WB analysis using 20456-1-AP (same clone as 20456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20456-1-AP (EI24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20456-1-PBS bindet in WB, IHC, Indirect ELISA EI24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EI24 fusion protein Ag14169
• Vollständiger Name: etoposide induced 2.4 mRNA
• Berechnetes Molekulargewicht: 340 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC002390
• Gene symbol: EI24
• Gene ID (NCBI): 9538
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).