EID1 Polyklonaler Antikörper

EID1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11734-1-AP

Synonyme

21 kDa pRb associated protein, C15orf3, CRI1, EID 1, EID1, IRO45620, PNAS 22, PTD014, RBP21

---

Galerie der Validierungsdaten

• 

  WB analysis using 11734-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11734-1-AP (EID1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11734-1-AP (EID1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11734-1-AP (EID1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11734-1-AP

  Immunofluorescent analysis of HepG2 cells using 11734-1-AP (EID1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11734-1-AP bindet in WB, IHC, IF, ELISA EID1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EID1 fusion protein Ag2336
• Vollständiger Name: EP300 interacting inhibitor of differentiation 1
• Berechnetes Molekulargewicht: 187 aa, 21 kDa
• Beobachtetes Molekulargewicht: 20-30 kDa
• GenBank-Zugangsnummer: BC024151
• Gene symbol: EID1
• Gene ID (NCBI): 23741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EID1(EP300 interacting inhibitor of differentiation 1) is a member of the EID protein family, which regulates differentiation, transcription and acetyltransferase activity. EID1 function was originally identified as a transcriptional repressor which regulates cell cycle and differentiation, and leads to genome inactivation and instability by interacting with E3 ubiquitin ligase MDM2. EID1 is mainly located in the cytoplasm. It was translocated into the nuclei under stressed or pathological conditions such as AD patients and APP Tg mice brains.(PMID: 22186421, PMID: 31381951,PMID: 30926163).

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; A comprehensive method for detecting ubiquitinated substrates using TR-TUBE.; Authors - Yukiko Yoshida
human	WB	Mol Cell Proteomics; Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.; Authors - Hua Liao
human	WB,IHC	Folia Histochem Cytobiol; EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells.; Authors - Ying Li