EIF1 Monoklonaler Antikörper

EIF1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G8C6

Kat-Nr. : 67601-1-Ig

Synonyme

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

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  WB analysis of HEK-293 using 67601-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67601-1-Ig (EIF1 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67601-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67601-1-Ig (EIF1 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67601-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67601-1-Ig (EIF1 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67601-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1 antibody (67601-1-Ig, Clone: 2G8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67601-1-Ig bindet in WB, IF/ICC, ELISA EIF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF1 fusion protein Ag7843
• Vollständiger Name: eukaryotic translation initiation factor 1
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC005118
• Gene symbol: EIF1
• Gene ID (NCBI): 10209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.