EIF1AD Polyklonaler Antikörper

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• KD/KO Validated

EIF1AD Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20528-1-AP

Synonyme

EIF1AD, Haponin

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 20528-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20528-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 20528-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20528-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, HEK-293-Zellen, HL-60-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

20528-1-AP bindet in WB, IF, ELISA EIF1AD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF1AD fusion protein Ag14390
• Vollständiger Name: eukaryotic translation initiation factor 1A domain containing
• Berechnetes Molekulargewicht: 165 aa, 19 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC005131
• Gene symbol: EIF1AD
• Gene ID (NCBI): 84285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Nature; Structural basis for the final steps of human 40S ribosome maturation.; Authors - Michael Ameismeier ; KD Validated
human	WB	Elife; The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.; Authors - Laura Plassart
human	WB	Elife; Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36.; Authors - Jasmin van den Heuvel
human	WB	Life Sci Alliance; CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau; Authors - Juan Carlos Polanco