EIF1AX Polyklonaler Antikörper

EIF1AX Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14654-1-PBS

Synonyme

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  WB analysis using 14654-1-AP (same clone as 14654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 14654-1-AP (same clone as 14654-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14654-1-AP (same clone as 14654-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF1AX antibody (14654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14654-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14654-1-PBS bindet in WB, IF/ICC, Indirect ELISA EIF1AX und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF1AX fusion protein Ag6253
• Vollständiger Name: eukaryotic translation initiation factor 1A, X-linked
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16-23 kDa
• GenBank-Zugangsnummer: BC067851
• Gene symbol: EIF1AX
• Gene ID (NCBI): 1964
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.