EIF2AK2, PKR Monoklonaler Antikörper

EIF2AK2, PKR Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A8A4

Kat-Nr. : 66646-1-Ig

Synonyme

EIF2AK2, PKR, 2A8A4, EC:2.7.10.2, EC:2.7.11.1

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  WB analysis using 66646-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66646-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66646-1-Ig bindet in WB, IHC, ELISA EIF2AK2, PKR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF2AK2, PKR fusion protein Ag13010
• Vollständiger Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Berechnetes Molekulargewicht: 551 aa, 62 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC093676
• Gene symbol: EIF2AK2
• Gene ID (NCBI): 5610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; The impact of single-stranded RNAs on the dimerization of double-stranded RNA-dependent protein kinase PKR; Authors - Tomoya Kitano