EIF2B2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF2B2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11034-1-AP

Synonyme

EIF 2Bbeta, EIF2B, EIF2B2, EIF2BB, S20I15, S20III15

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Galerie der Validierungsdaten

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  WB analysis using 11034-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11034-1-AP (EIF2B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11034-1-AP

  IP result of anti-EIF2B2 (IP:11034-1-AP, 4ug; Detection:11034-1-AP 1:500) with HEK-293 cells lysate 2000 ug.

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  IHC staining of rat brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11034-1-AP (EIF2B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, K-562-Zellen, Mauslebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

11034-1-AP bindet in WB, IP, IHC, IF, ELISA EIF2B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF2B2 fusion protein Ag1502
• Vollständiger Name: eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC011750
• Gene symbol: EIF2B2
• Gene ID (NCBI): 8892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Elife; Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.; Authors - Carmela Sidrauski
	WB	Elife; The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.; Authors - Yao Liang Wong
human	WB	Elife; eIF2B conformation and assembly state regulate the integrated stress response.; Authors - Michael Schoof
human	WB	Elife; A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation.; Authors - Morgane Boone
human	WB	RNA; EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response.; Authors - Stephanie L Moon
human	WB	Biochem J; Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.; Authors - Noel C Wortham ; KD Validated