EIF2B5 Polyklonaler Antikörper

EIF2B5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29935-1-PBS

Synonyme

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  WB analysis using 29935-1-AP (same clone as 29935-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29935-1-AP (EIF2B5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29935-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EIF2B5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF2B5 fusion protein Ag32175
• Vollständiger Name: eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 65-80 kDa
• GenBank-Zugangsnummer: NM_003907.2
• Gene symbol: EIF2B5
• Gene ID (NCBI): 8893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).