EIF2C3-Specific Polyklonaler Antikörper

EIF2C3-Specific Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19692-1-AP

Synonyme

AGO3, Argonaute 3, Argonaute3, eIF 2C 3, eIF2C 3, EIF2C3, hAgo3, Protein argonaute 3

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 19692-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

19692-1-AP bindet in WB,ELISA EIF2C3-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2C, 3
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_024852
• Gene symbol: EIF2C3
• Gene ID (NCBI): 192669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2C3, also named as AGO3, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi). EIF2C3 binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. EIF2C3 lacks endonuclease activity and does not appear to cleave target mRNAs. This antibody is specific to EIF2C3.

Publikationen

Species	Application	Title
human	WB	Nat Struct Mol Biol; A novel class of microRNA-recognition elements that function only within open reading frames.; Authors - Kai Zhang