EIF2S1 Monoklonaler Antikörper

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• KD/KO Validated

EIF2S1 Monoklonal Antikörper für WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C8E2

Kat-Nr. : 68479-1-PBS

Synonyme

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

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  WB analysis using 68479-1-Ig (same clone as 68479-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68479-1-Ig (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  WB result of EIF2S1 antibody (68479-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2S1 transfected HepG2 cells. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  IP result of anti-EIF2S1 (IP:68479-1-Ig, 4ug; Detection:68479-1-Ig 1:4000) with HepG2 cells lysate 1480 ug. This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68479-1-Ig (same clone as 68479-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (68479-1-Ig, Clone: 3C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68479-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50181-1

  Cytometric bead array in cell lysate using MP50181-1, Phospho-EIF2S1 (Ser51) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68479-1-PBS. Detection antibody: 68023-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Geprüfte Anwendungen

Produktinformation

68479-1-PBS bindet in WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA EIF2S1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF2S1 fusion protein Ag28309
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002513
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.