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EIF2S2 Monoklonaler Antikörper

EIF2S2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H8E1

Kat-Nr. : 68463-1-PBS

Synonyme

2H8E1, eIF 2 beta, EIF2, EIF2B, EIF2beta

Filter:
  • Western Blot (WB) analysis of various lysates using EIF2S2 Monoclonal antibody (68463-1-Ig)

    WB analysis using 68463-1-Ig (same clone as 68463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68463-1-Ig (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68463-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68463-1-PBS bindet in WB, Indirect ELISA EIF2S2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EIF2S2 fusion protein Ag18349
Vollständiger Name eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
Berechnetes Molekulargewicht 38 kDa
Beobachtetes Molekulargewicht50 kDa
GenBank-ZugangsnummerBC000461
Gene symbol EIF2S2
Gene ID (NCBI) 8894
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.