EIF3A Polyklonaler Antikörper

EIF3A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 26178-1-AP

Synonyme

eIF3 p185, eIF3 p180, eIF3 p170, eIF3 p167, EIF3

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  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26178-1-AP (EIF3A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 26178-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26178-1-AP (EIF3A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 26178-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF3A antibody (26178-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

26178-1-AP bindet in WB, IHC, IF/ICC, RIP, ELISA EIF3A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3A fusion protein Ag22515
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit A
• Berechnetes Molekulargewicht: 1382 aa, 166 kDa
• Beobachtetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: BC114429
• Gene symbol: EIF3A
• Gene ID (NCBI): 8661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
human	WB,IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo
human	WB	Onco Targets Ther; WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.; Authors - Jingjing Dai
mouse	WB	J Clin Lab Anal; Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.; Authors - Zhenwei Li
human	RIP	Proc Natl Acad Sci U S A; Epitranscriptic regulation of HRAS by N6-methyladenosine drives tumor progression; Authors - Yongbo Pan
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi