EIF3B Monoklonaler Antikörper

EIF3B Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12G12

Kat-Nr. : 68202-1-PBS

Synonyme

eIF 3 eta, EIF3 ETA, EIF3 P110, EIF3 P116, EIF3B, EIF3S9, hPrt1, PRT1, Prt1 homolog

---

• 

  WB analysis using 68202-1-Ig (same clone as 68202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68202-1-Ig (EIF3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68202-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68202-1-PBS bindet in WB, Indirect ELISA EIF3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF3B fusion protein Ag31427
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit B
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC001173
• Gene symbol: EIF3B
• Gene ID (NCBI): 8662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF3B, also named Eukaryotic translation initiation factor 3 subunit B, is an 814 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and 8 WD repeats and belongs to the eIF-3 subunit B family. EIF3B as an RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation, and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. The calculated molecular weight of EIF3B is 93 kDa, but the phosphorylated EIF3B protein is about 115 kDa.