EIF3D Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF3D Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hefe, human, Maus und mehr (2)

Anwendung

WB, PLA, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (3)

Kat-Nr. : 10219-1-AP

Synonyme

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10219-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10219-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10219-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10219-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 10219-1-AP

  IP result of anti-EIF3D (IP:10219-1-AP, 4ug; Detection:10219-1-AP 1:300) with A549 cells lysate 2800 ug.

• 

  IHC staining of human breast cancer using 10219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10219-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining using 10219-1-AP

  IF result of 10219-1-AP (anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha.U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3D antibody (10219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of transfected cells using 10219-1-AP

  IF result of 10219-1-AP (anti-EIF3D) in U2OS cell by Dr. Nancy Kedersha. U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with 10219-1-AP (EIF3D) in green; and counterstained with Santa Cruz anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.; The EIF3D(p66) appears associated with mitochondria.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 10219-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of MCF-7 using 10219-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF3D antibody 10219-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, L02-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, HepG2-Zellen, MCF-7-Zellen, mit Ethacrynsäure behandelte HepG2-Zellen, Transfizierte Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10219-1-AP bindet in WB, PLA, IP, IHC, IF, CoIP, ELISA EIF3D und zeigt Reaktivität mit Hefe, human, Maus

• Getestete Reaktivität: Hefe, human, Maus
• In Publikationen genannte Reaktivität: human, Hefe, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3D fusion protein Ag0268
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit D
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC000328
• Gene symbol: EIF3D
• Gene ID (NCBI): 8664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.; Authors - Zhen Shi
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human	IHC	EBioMedicine; PMID: 31669222; Authors - Hai Huang
mouse	WB	Dev Cell; Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c; Authors - Kotaro Fujii
rabbit	WB	EMBO J; HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.; Authors - Locker Nicolas N
human	WB	PLoS Pathog; A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.; Authors - Eoin N Leen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tobias (Verified Customer) (10-25-2022)

clean and good antibody for wb

• Applications: Western Blot

FH

Peter (Verified Customer) (10-24-2022)

Specific band at correct MW

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549

FH

Joleen (Verified Customer) (06-10-2019)

EIF3D antibody recognizes a prominent band around the correct molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

EIF3D Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10219-1-AP)