EIF3E Polyklonaler Antikörper

EIF3E Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 10899-1-AP

Synonyme

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

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  WB analysis of rat lymph using 10899-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10899-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10899-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10899-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10899-1-AP

  IP result of anti-EIF3E (IP:10899-1-AP, 4ug; Detection:10899-1-AP 1:500) with Jurkat cells lysate 2440 ug.

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  IHC staining of human pancreas cancer using 10899-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of U2OS using 10899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 10899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlymphgewebe, HeLa-Zellen, Jurkat-Zellen, Maus-Pankreasgewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10899-1-AP bindet in WB, IHC, IF, IP, ELISA EIF3E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3E fusion protein Ag1132
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit E
• Berechnetes Molekulargewicht: 445 aa, 52 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC008419
• Gene symbol: EIF3E
• Gene ID (NCBI): 3646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Aging Cell; Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells.; Authors - Yang You
human	IHC	Cancer Genomics Proteomics; Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate; Authors - MOHAMED E. M. SAEED
human	WB	Cancer Lett; N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination; Authors - Chengquan Shen
pig	WB	J Virol; VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1; Authors - Lu Li