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EIF3E Monoklonaler Antikörper

EIF3E Monoklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6C8

Kat-Nr. : 67095-1-PBS

Synonyme

1C6C8, eIF-3 p48, EIF3S6, INT6, Viral integration site protein INT-6 homolog

Filter:
  • Western Blot (WB) analysis of various lysates using EIF3E Monoclonal antibody (67095-1-Ig)

    WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF3E Monoclonal antibody (67095-1-Ig)

    IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3E Monoclonal antibody (67095-1-Ig)

    IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67095-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA EIF3E und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EIF3E fusion protein Ag28523
Vollständiger Name eukaryotic translation initiation factor 3, subunit E
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48-52 kDa
GenBank-ZugangsnummerBC000734
Gene symbol EIF3E
Gene ID (NCBI) 3646
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.