EIF3G Polyklonaler Antikörper

EIF3G Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 11165-1-AP

Synonyme

eIF 3 delta, eIF 3 RNA binding subunit, eIF3 delta, eIF3 p42, eIF3 p44

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  WB analysis of HepG2 using 11165-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11165-1-AP (EIF3G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11165-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11165-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3G antibody (11165-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of A549 using 11165-1-AP

  Immunofluorescent analysis of A549 cells, using EIF3G antibody 11165-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

11165-1-AP bindet in WB, IHC, IF/ICC, ELISA EIF3G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3G fusion protein Ag1653
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit G
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000733
• Gene symbol: EIF3G
• Gene ID (NCBI): 8666
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3G is a component of the EIF3 complex. [PMID:20679478,20503360]

Publikationen

Species	Application	Title
mouse	WB	Nat Chem Biol; Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.; Authors - Xin Erica Shu
human	IF	PLoS Pathog; The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation.; Authors - Jordan M Meyers
human	IF	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas
		Cell Rep; N6-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism; Authors - Binbin Chen