EIF3J Polyklonaler Antikörper

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• KD/KO Validated

EIF3J Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10439-1-PBS

Synonyme

Eukaryotic translation initiation factor 3 subunit 1, EIF3S1, eIF-3-alpha, eIF3 p35, eIF3 alpha

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  WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10439-1-AP (same clone as 10439-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10439-1-AP (same clone as 10439-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10439-1-AP (same clone as 10439-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10439-1-AP (same clone as 10439-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 10439-1-AP (same clone as 10439-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 10439-1-AP (same clone as 10439-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  IP result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3J antibody (10439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10439-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA EIF3J und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF3J fusion protein Ag0678
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit J
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC002719
• Gene symbol: EIF3J
• Gene ID (NCBI): 8669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

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