EIF3M Monoklonaler Antikörper

EIF3M Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A12B2

Kat-Nr. : 66025-1-PBS

Synonyme

PCID1, 2A12B2, B5, EIF3, GA17

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  WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66025-1-Ig (same clone as 66025-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66025-1-Ig (same clone as 66025-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF3M antibody (66025-1-Ig, Clone: 2A12B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using EIF3M antibody 66025-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66025-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EIF3M und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF3M fusion protein Ag17965
• Vollständiger Name: eukaryotic translation initiation factor 3, subunit M
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• Beobachtetes Molekulargewicht: 35-43 kDa
• GenBank-Zugangsnummer: BC019103
• Gene symbol: EIF3M
• Gene ID (NCBI): 10480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.