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EIF4A2 Monoklonaler Antikörper

EIF4A2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11E11

Kat-Nr. : 67799-1-PBS

Synonyme

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

Filter:
  • Western Blot (WB) analysis of various lysates using EIF4A2 Monoclonal antibody (67799-1-Ig)

    WB analysis using 67799-1-Ig (same clone as 67799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EIF4A2 Monoclonal antibody (67799-1-Ig)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67799-1-PBS bindet in WB, IF/ICC, Indirect ELISA EIF4A2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen EIF4A2 fusion protein Ag9085
Vollständiger Name eukaryotic translation initiation factor 4A, isoform 2
Berechnetes Molekulargewicht 408 aa, 46 kDa
Beobachtetes Molekulargewicht46 kDa
GenBank-ZugangsnummerBC012547
Gene symbol EIF4A2
Gene ID (NCBI) 1974
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.