EIF4E3 Monoklonaler Antikörper

EIF4E3 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E9C4

Kat-Nr. : 68171-1-PBS

Synonyme

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

---

• 

  WB analysis using 68171-1-Ig (same clone as 68171-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68171-1-Ig (EIF4E3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 68171-1-Ig (same clone as 68171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68171-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68171-1-PBS bindet in WB, IHC, Indirect ELISA EIF4E3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF4E3 fusion protein Ag11056
• Vollständiger Name: eukaryotic translation initiation factor 4E family member 3
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-30 kDa
• GenBank-Zugangsnummer: BC031289
• Gene symbol: EIF4E3
• Gene ID (NCBI): 317649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918).