EIF4G1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF4G1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 15704-1-AP

Synonyme

eIF-4G 1, EIF4G, EIF4F, eIF 4G1, eIF 4G 1

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  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15704-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15704-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15704-1-AP

  IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15704-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4G1 antibody (15704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 15704-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, mit Ethacrynsäure behandelte HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15704-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA EIF4G1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4G1 fusion protein Ag8342
• Vollständiger Name: eukaryotic translation initiation factor 4 gamma, 1
• Berechnetes Molekulargewicht: 1600 aa, 176 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC007788
• Gene symbol: EIF4G1
• Gene ID (NCBI): 1981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Publikationen

Species	Application	Title
	CoIP	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	IF	J Extracell Vesicles; Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.; Authors - Bo You
human	WB	Proc Natl Acad Sci U S A; Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.; Authors - Brian C Avanzino
human	WB	PLoS Genet; SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.; Authors - Siqi Hu
mouse	WB,IF	Diabetes; Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.; Authors - Seokwon Jo ; KO Validated
human	WB	Virol Sin; Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A; Authors - Yanyan Dong