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EIF4G3 Polyklonaler Antikörper

EIF4G3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11281-1-PBS

Synonyme

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

Filter:
  • Western Blot (WB) analysis of various lysates using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis of HEK-293 using 11281-1-AP (same clone as 11281-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using EIF4G3 Polyclonal antibody (11281-1-AP)

    IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11281-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA EIF4G3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EIF4G3 fusion protein Ag1811
Vollständiger Name eukaryotic translation initiation factor 4 gamma, 3
Berechnetes Molekulargewicht 177 kDa
Beobachtetes Molekulargewicht 70 kDa, 250 kDa
GenBank-ZugangsnummerBC030578
Gene symbol EIF4G3
Gene ID (NCBI) 8672
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.