EIF5A Polyklonaler Antikörper

EIF5A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11309-1-AP

Synonyme

eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, EIF5A, EIF5A1, eIF5AI, Rev binding factor

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Galerie der Validierungsdaten

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  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11309-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF5A antibody (11309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11309-1-AP

  Immunofluorescent analysis of HeLa cells using 11309-1-AP (EIF5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11309-1-AP bindet in WB, IHC, IF, ELISA EIF5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF5A fusion protein Ag1849
• Vollständiger Name: eukaryotic translation initiation factor 5A
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001832
• Gene symbol: EIF5A
• Gene ID (NCBI): 1984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

Publikationen

Species	Application	Title
human	WB,IHC	J Cancer; EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma.; Authors - Linjing Li
mouse	IF	J Ovarian Res; Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes; Authors - Chuanxin Zhang
human	WB	Oncol Lett; SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A; Authors - Boya Deng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated