EIF5A Monoklonaler Antikörper

EIF5A Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D1B6

Kat-Nr. : 67214-1-PBS

Synonyme

2D1B6, eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1

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  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67214-1-Ig (same clone as 67214-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67214-1-PBS bindet in WB, IF/ICC, Indirect ELISA EIF5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF5A fusion protein Ag28588
• Vollständiger Name: eukaryotic translation initiation factor 5A
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC001832
• Gene symbol: EIF5A
• Gene ID (NCBI): 1984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.