EIF5B Polyklonaler Antikörper

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• KD/KO Validated

EIF5B Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13527-1-PBS

Synonyme

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  WB analysis of mouse brain using 13527-1-AP (same clone as 13527-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13527-1-AP (same clone as 13527-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 13527-1-AP (same clone as 13527-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13527-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EIF5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF5B fusion protein Ag4404
• Vollständiger Name: eukaryotic translation initiation factor 5B
• Berechnetes Molekulargewicht: 1220 aa, 139 kDa
• Beobachtetes Molekulargewicht: 175 kDa
• GenBank-Zugangsnummer: BC032639
• Gene symbol: EIF5B
• Gene ID (NCBI): 9669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).