EIF6 Rekombinanter Antikörper

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• KD/KO Validated

EIF6 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G23

Kat-Nr. : 82693-1-PBS

Synonyme

integrin beta 4 binding protein, 2G23, b, b(2)gcn, B(2)GCN homolog

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  WB analysis using 82693-1-RR (same clone as 82693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82693-1-RR (EIF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82693-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82693-1-RR (same clone as 82693-1-PBS)

  WB result of EIF6 antibody (82693-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF6 transfected HeLa cells. This data was developed using the same antibody clone with 82693-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82693-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82693-1-RR against Human EIF6 were performed. The affinity constant is 6.29 nM.

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Geprüfte Anwendungen

Produktinformation

82693-1-PBS bindet in WB, Indirect ELISA EIF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EIF6 fusion protein Ag0324
• Vollständiger Name: eukaryotic translation initiation factor 6
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC001119
• Gene symbol: EIF6
• Gene ID (NCBI): 3692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.