ELAC2 Polyklonaler Antikörper

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• KD/KO Validated

ELAC2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10071-1-AP

Synonyme

elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2

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Galerie der Validierungsdaten

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  WB analysis of DU 145 using 10071-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10071-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10071-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10071-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10071-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Produktinformation

10071-1-AP bindet in WB, IHC, IF, ELISA ELAC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELAC2 fusion protein Ag0105
• Vollständiger Name: elaC homolog 2 (E. coli)
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC004158
• Gene symbol: ELAC2
• Gene ID (NCBI): 60528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	IF	Cell Metab; GRSF1 regulates RNA processing in mitochondrial RNA granules.; Authors - Jourdain Alexis A AA
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen
human	WB	Nat Commun; Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.; Authors - Zbigniew Pietras
mouse	WB	Cell Rep; Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.; Authors - Oliver Rackham
human	WB,IF	RNA Biol; Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs.; Authors - Brzezniak Lien K LK ; KD Validated