HuA/B/C/D Polyklonaler Antikörper

HuA/B/C/D Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13032-1-AP

Synonyme

ELAV like protein 4, ELAVL4, Hu antigen D, Hu C/D, HuA/B/C/D, HUD, PNEM

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Galerie der Validierungsdaten

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  WB analysis of human cerebellum using 13032-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 13032-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 13032-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13032-1-AP (HuA/B/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13032-1-AP

  IP Result of anti-HuA/B/C/D (IP:13032-1-AP, 4ug; Detection:13032-1-AP 1:300) with mouse brain tissue lysate 2560ug.

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 13032-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13032-1-AP (HuA/B/C/D antibody at dilution of 1:200 (under 10x lens).

• 

  IF Staining of mouse brain using 13032-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuA/B/C/D antibody (13032-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Cerebellum-Gewebe, Maus-Cerebellum-Gewebe, PC-13-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13032-1-AP bindet in WB, IP, IHC, IF, ELISA HuA/B/C/D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HuA/B/C/D fusion protein Ag3678
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Berechnetes Molekulargewicht: 380 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC036071
• Gene symbol: ELAVL4
• Gene ID (NCBI): 1996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].

Publikationen

Species	Application	Title
rat	IHC	J Neurosci Methods; Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF.; Authors - Ruth Butler-Ryan
human	IHC,WB	J Cosmet Dermatol; Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging; Authors - Juping Chen
mouse	IF	Med Mol Morphol; Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice; Authors - Nami O Yamada
mouse	IF	Biomed Pharmacother; Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis; Authors - Jinzhao Bai