HuD-specific Polyklonaler Antikörper

HuD-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24992-1-AP

Synonyme

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, HuD-specific, PNEM

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 24992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24992-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24992-1-AP (HuD-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 24992-1-AP

  IP Result of anti-HuD-specific (IP:24992-1-AP, 4ug; Detection:24992-1-AP 1:300) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human brain using 24992-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 24992-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24992-1-AP (HuD-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 24992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using HuD-specific antibody (24992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 24992-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24992-1-AP (HuD-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

24992-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA HuD-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HuD-specific fusion protein Ag21830
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Berechnetes Molekulargewicht: 380 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC036071
• Gene symbol: ELAVL4
• Gene ID (NCBI): 1996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 is expressed in brain and localizes in the nucleus. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Publikationen

Species	Application	Title
human	IF	Acta Neuropathol; Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.; Authors - Sandra Diaz-Garcia
human	WB	Prog Neurobiol; RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons.; Authors - Robert J van der Linden
human	WB,RIP	FASEB J; ELAVL4 promotes the tumorigenesis of small cell lung cancer by stabilizing LncRNA LYPLAL1-DT and enhancing profilin 2 activation; Authors - Shuxin Li