ELL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ELL2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 12727-1-AP

Synonyme

ELL 2

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• 

  WB analysis using 12727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12727-1-AP

  IP result of anti-ELL2 (IP:12727-1-AP, 4ug; Detection:12727-1-AP 1:300) with mouse brain tissue lysate 3440ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen, Maushirngewebe, Maus-Magengewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12727-1-AP bindet in WB, IF, IP, ChIP, ELISA ELL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELL2 fusion protein Ag3428
• Vollständiger Name: elongation factor, RNA polymerase II, 2
• Berechnetes Molekulargewicht: 640 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC028412
• Gene symbol: ELL2
• Gene ID (NCBI): 22936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998).

Publikationen

Species	Application	Title
mouse	WB	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	ChIP	Cell Death Dis; TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response; Authors - Xinyue Sun
mouse	WB	J Biol Chem; Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells.; Authors - Yuanzheng Peng
human	WB	FASEB J; Androgen receptor splicing variant 7 (ARv7) promotes DNA damage response in prostate cancer cells; Authors - Haoge Luo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Beth (Verified Customer) (10-05-2017)

Using LI-COR imaging system.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RWPE1, PC-3

ELL2 Antibody Western Blot validation (1:500 dilution) in RWPE1, PC-3 (Cat no:12727-1-AP)