ELOVL4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ELOVL4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 55023-1-AP

Synonyme

STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD

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  WB analysis using 55023-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 55023-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55023-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55023-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 55023-1-AP

  Immunofluorescent analysis of A431 cells using 55023-1-AP (ELOVL4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, A431-Zellen, Maushirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Dünndarmgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

55023-1-AP bindet in WB, IHC, IF/ICC, ELISA ELOVL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: NM_022726
• Gene symbol: ELOVL4
• Gene ID (NCBI): 6785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling; Authors - Yanchuan Li ; KD Validated
human	WB	Ocul Surf; PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).; Authors - Sun Woong Kim
mouse	IHC	Ocul Surf; Ocular surface alkali injury damages meibomian glands in mice.; Authors - Shin Mizoguchi
mouse	WB	Int J Mol Sci; In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism.; Authors - Nesli-Ece Sen
rat	WB	Invest Ophthalmol Vis Sci; Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway.; Authors - Peng Zhang
human	WB	Lipids; Phytosphingosine Increases Biosynthesis of Phytoceramide by Uniquely Stimulating the Expression of Dihydroceramide C4-desaturase (DES2) in Cultured Human Keratinocytes.; Authors - Hyun Kyung Choi