EMB Polyklonaler Antikörper

EMB Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13946-1-PBS

Synonyme

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  WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 13946-1-AP (same clone as 13946-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13946-1-PBS bindet in WB, IHC, Indirect ELISA EMB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EMB fusion protein Ag5031
• Vollständiger Name: embigin homolog (mouse)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC059398
• Gene symbol: EMB
• Gene ID (NCBI): 133418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)