Emerin Rekombinanter Antikörper

Emerin Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230089C4

Kat-Nr. : 82888-2-PBS

Synonyme

EMD, 230089C4, EDMD, LEMD5, STA

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  IHC staining of human thyroid cancer using 82888-2-RR (same clone as 82888-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 82888-2-RR (same clone as 82888-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human Emerin (82888-2-RR, Clone:230089C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82888-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-2-RR against Human Emerin were performed. The affinity constant is 1.71 nM.

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Geprüfte Anwendungen

Produktinformation

82888-2-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA Emerin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Emerin fusion protein Ag0236
• Vollständiger Name: emerin
• Berechnetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.