Emerin Rekombinanter Antikörper

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Emerin Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230089B12

Kat-Nr. : 82888-3-PBS

Synonyme

EMD, 230089B12, EDMD, LEMD5, STA

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  WB analysis using 82888-3-RR (same clone as 82888-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82888-3-RR (same clone as 82888-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82888-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

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Geprüfte Anwendungen

Produktinformation

82888-3-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA Emerin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Emerin fusion protein Ag0236
• Vollständiger Name: emerin
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.